Studies on Serum Lipoprotein Abnormalities with Polyacrylamide Gel Electrophoresis and Preparative and Analytical Ultracentrifuge:Studies on the Two Kinds of the Midband Lipoproteins

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概要

• 論文の詳細を見る

• Two kinds of the midband lipoproteins (Lps) were studied. The one was a midband which occurred very closely to β Lp and appeared as a double-β Lp profile on polyacrylamide gel (PAG) disc electrophoresis. The other was those appeared as the braod-midband Lp profile, which occurred frequently in uremic patients on hemodialysis.<BR>The midband Lp of the double-β Lp-emia was detected not in LDL which migrates at β Lp position usually but in HDL (d 1.063-1.21) which migrates at α Lp position usually, exclusively (HD-midband Lp). As isolated by PAG block electrophoresis, HD-midband Lp showed a lipid composition intermediate between β Lp and pre-β Lp. This HD-midband Lp occurred in 1.8% of a population of approximately 1100 and showed familial corroboration.<BR>LDLs isolated from the patients with the broad-midband Lp profile produced 2-3 Lp bands on PAG block electrophoresis and 2-3 peaks in ultracentrifugal Schlieren analysis. One of such LDLs which showed 3 Lp bands and 3 peaks was subjected to a series of ultracentrifugal analyses to determine buoyant densities of each peak-component. On the basis of these analyses, the 3 peak-components were isolated ultracentrifugally and it was revealed that they are consisted of Sf 13, 10, 7 and 6 LDL-subfractions. These LDL-subfractions demonstrated electrophoretic behaviors and chemical compositions intermediate between pre-β-VLDL and β-LDL, suggesting abnormal accumulation of catabolic remnant(s) of VLDL.<BR>It was concluded, at least tentatively, that for the most adequate identification of abnormal Lps which appear as the midb and Lps and may have atherogenic potential or any other pathological significance, which is obviously the primary purpose of such study as this, carefull characterization of the midband Lps seemed to be essential and productive.

• Japan Society of Clinical Chemistryの論文

著者

• 三瀬 淳一

  山口大学医学部三瀬内科

• 和田 光夫

  山口大学医学部 三瀬内科

• 岡部 光久

  山口大学医学部第二内科

• 薦田 信

  山口大学医学部第二内科

• 南園 忠

  山口大学医学部第二内科

• 三瀬 淳一

  山口大学医学部第二内科

• 三瀬 淳一

  山口大学医学部

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