Luminol-dependent chemiluminescence of polymorphonuclear leukocytes from patients with gout

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We investigated luminol - dependent chemiluminescence (LDCL) of peripheral blood PMNs from gouty patients. The reaction mixture consisted of PMNs, luminol solution and synthetic monosodium urate (MSU) crystals.<BR>MSU crystals and inactivated serum-treated MSU crystals did not generate LDCL of PMNs from normal individuals and gouty patients, but fresh serumtreated MSU crystals did. In acute gouty arthritis, LDCL of PMNs was enhanced more prominently than in patients without attacks whose LDCL showed higher levels than those of normal individuals. Even in patients with acute attacks of gouty arthritis administered colchicine, LDCL of PMNs was prominently suppressed by MSU. Moreover, compared to LDCL induced by opsonized zymosan, LDCL of PMNs from patients with acute gouty arthritis showed low levels.<BR>The present study demonstrates that MSU crystals treated with fresh serum evoked LDCL of peripheral blood PMNs in gouty patients.