Simultaneous Measurement of Serotonin, Dopamine and Their Metabolites in Mouse Brain Extracts by High-Performance Liquid Chromatography with Mass Spectrometry following Derivatization with Ethyl Chloroformate
スポンサーリンク
概要
- 論文の詳細を見る
In order to measure the levels of serotonin (5-hydroxyltryptamine, 5-HT), dopamine (DA), 3,4-hydroxyphenylacetic acid (DOPAC), 3-methoxytyramine (3-MT) and homovanillic acid (HVA) simultaneously, an effective derivatization method followed by high-performance liquid chromatography (HPLC) coupled to electrochemical ionization mass spectrometry was used. The derivatization reaction of biological samples with ethyl chloroformate occurred rapidly at room temperature in aqueous conditions, and the resulting derivatives were isocratically separated with good selectivity using a C18 reversed-phase column within 30 min. The study results showed that the new derivatization procedure offers an excellent means of simultaneous determination of 5-HT, DA and their metabolites in mouse brain homogenates, which are important in a number of physiological and behavioral functions.
著者
-
Myung Seung-woon
Department Of Chemistry Kyonggi University
-
Park Ju-young
Department Of Global Agricultural Sciences The University Of Tokyo
-
Kwon Soon-jung
Department Of Architecture Ajou University
-
Yoon Sung-Hwa
Department of Molecular Science and Technology, Ajou University
-
Choi Dong-kug
Department Of Biotechnology Konkuk University
-
Kim In-soo
Department Of Neurosurgery Myong-ji St. Mary's Hospital
-
CHOI Dong-Kug
Department of Biotechnology, Konkuk University
-
Kwon Soon-Jung
Department of Molecular Science and Technology, Ajou University
-
Kim In-Soo
Department of Biotechnology, Konkuk University
関連論文
- Screening of fungal antagonists against yellows of cabbage caused by Fusarium oxysporum f. sp. conglutinans
- Gas chromatograph-mass spectrometric method for the determination of carvedilol and its metabolites in human urine
- Heterocyclic Amines in Several Types of Cooked Meat and Chicken Dishes which Form Part of the Korean Diet
- Simplified Heterocyclic Analogues of Fluoxetine Inhibit Inducible Nitric Oxide Production in Lipopolysaccharide-Induced BV2 Cells
- Anti-neuroinflammatory Activity of Kamebakaurin From Isodon japonicus via Inhibition of c-Jun NH2-Terminal Kinase and p38 Mitogen-Activated Protein Kinase Pathway in Activated Microglial Cells
- MMHD [(S,E)-2-Methyl-1-(2-methylthiazol-4-yl) hexa-1,5-dien-ol], a Novel Synthetic Compound Derived From Epothilone, Suppresses Nuclear Factor-κB–Mediated Cytokine Expression in Lipopolysaccharide-Stimulated BV-2 Microglia
- A Study on Characteristics of Subway Utilization and Pedestrians' Accessibility at New Towns in Korea(Architectural/Urban Planning and Design)
- MMHD [(S,E)-2-Methyl-1-(2-methylthiazol-4-yl) hexa-1,5-dien-ol], a Novel Synthetic Compound Derived From Epothilone, Suppresses Nuclear Factor-κB-Mediated Cytokine Expression in Lipopolysaccharide-Stimulated BV-2 Microglia
- Anti-neuroinflammatory Activity of Kamebakaurin From Isodon japonicus via Inhibition of c-Jun NH_2-Terminal Kinase and p38 Mitogen-Activated Protein Kinase Pathway in Activated Microglial Cells
- Surgical Treatment of Moyamoya Disease in Adults : Combined Direct and Indirect vs. Indirect Bypass Surgery
- Cognitive Enhancing Effects of Alpha Asarone in Amnesic Mice by Influencing Cholinergic and Antioxidant Defense Mechanisms
- Simultaneous Measurement of Serotonin, Dopamine and Their Metabolites in Mouse Brain Extracts by High-Performance Liquid Chromatography with Mass Spectrometry following Derivatization with Ethyl Chloroformate
- Anti-neuroinflammatory Activity of a Novel Cannabinoid Derivative by Inhibiting the NF-κB Signaling Pathway in Lipopolysaccharide-Induced BV-2 Microglial Cells