STIMULATING EFFECTS OF ALANINE AND GLYCINE ON GUINEA-PIG TAENIA COLI

概要

論文の詳細を見る
Effects of alanine and glycine on the mechanical and electrical activities of guinea-pig taenia coli were investigated. Alanine (0.1-10mM) and glycine (0.5-10mM) produced a does dependent contraction of guinea-pig taenia coli. The stimulating effects of alanine and glycine were not inhibited in the presence of atropine (5μM), tetrodotoxin (0.1μg/ml), diphenhydramine (4μM), methysergide (3μM), strychnine (10μM), and were not influenced by treatment with indomethacin (3μM). However, these effects were inhibited in the presence of a Ca antagonist, verapamil (10μM). When the electrical activities of the taenia coli were recorded by the single sucrose-gap method, alanine and glycine (5-10mM) produced a reduction of membrane potential and increased spike heights and frequencies of action potential. In LiCl or Choline-Cl and in Na-isethionate solutions, the stimulating effect of alanine was not abolished, but was completely inhibited in KCl-depolarized preparation.<BR>From these results, it is considered that both alanine and glycine may directly produce contraction by a depolarization of the cell membrane of guinea-pig taenia coli.
日本平滑筋学会の論文