Electric birefringence and electric dichroism of sonicated DNA in aqueous solutions with various additives. Electro-optical and hydrodynamic properties.
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概要
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Electric birefringence (EB) and electric linear dichroism (ED) of a sonicated DNA (<I>M</I><SUB>w</SUB>=1.24×10<SUP>5</SUP>) sample were studied in solutions containing various additives at 7 °C. Signals of EB at 535 nm and of ED at 260 nm were measured in the electric field range 0–<I>ca.</I> 19 kV/cm. Both signals obeyed the Kerr law at low field strengths (E<<I>ca.</I> 5 kV/cm) and showed a saturating trend at high fields. The mean relaxation time, <τ><SUB>EB</SUB>, obtained from the decay of EB, was field-strength dependent, indicating the effect of the polydispersity of sDNA. The field strength dependence of steady-state EB signals was nearly identical with that of ED signals. These results were analyzed with the "classical" orientation functions derived for mono- and polydisperse systems. The optical anisotropy factor (<I>g</I><SUB>3</SUB>–<I>g</I><SUB>1</SUB>) of sDNA was <I>ca.</I> −2.0×10<SUP>−2</SUP>, while the average angle between the orientation axis of sDNA and the transition moment of the base pair was <I>ca.</I> 70° at 260 nm. The electric moment of sDNA was influenced by ionic strength, counterions, and other additives. The weight-average length <I>l</I><SUB>w</SUB> of sDNA decreased in this order: 0.2 mM NaCl<SUP>**</SUP>>9-aminoacridinium chloride (<I>P</I>⁄<I>D</I>=10)>1.0 mM NaCl>0.33 mM MgCl<SUB>2</SUB>> 20 vol% glycerol. Values of <I>l</I><SUB>w</SUB>/base pair were 2.8−2.7 Å in the glycerol and 3.6−3.4 Å in the 1.0 mM NaCl.
- 公益社団法人 日本化学会の論文
著者
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Yamaoka Kiwamu
Faculty of Science, Hiroshima University
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Matsuda Koichiro
Faculty of Science, Hiroshima University
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