Effects of divalent metal cations on circular dichroism and 1H nuclear magnetic resonance spectra of linear and cyclic peptides having side-chain imidazolyl and acetamido groups.
スポンサーリンク
概要
- 論文の詳細を見る
A linear peptide, <I>N</I>-<I>t</I>-butoxycarbonyl-<I>S</I>-acetamidomethyl-L-cysteinyl-D-leucyl-L-histidyl-<I>S</I>-acetamidomethyl-L-cysteinyl-D-leucyl-L-histidine methyl ester (<B>1</B>) was synthesized by the solid phase method. Subsequent cyclization by the azide method offered a cyclic peptide, <I>cyclo</I>-<I>S</I>-acetamidomethyl-L-cysteinyl-D-leucyl-L-histidyl-<I>S</I>-acetamidomethyl-L-cysteinyl-D-leucyl-L-histidyl (<B>2</B>). The circular dichroism (CD) and <SUP>1</SUP>H NMR were observed for these peptides <B>1</B> and <B>2</B>, in the absence and presence of metal cations, Zn(II), Ca(II), Mg(II). Spectral results for Zn(II) complexes indicated striking contrasts to those of Ca(II) and Mg(II) complexes. Thus, in H<SUB>2</SUB>O, the addition of Zn(II) caused CD spectral changes for both peptides, while Ca(II) and Mg(II) did not cause changes in any CD spectral region. In methanol, the additions of Zn(II) and Ca(II) caused CD changes, while the addition of Mg(II) did not cause any CD changes. The changes caused by adding Zn(II) were quite different from those by Ca(II), suggesting that Zn(II) is coordinated with the peptides in a mode different from that of Ca(II). <SUP>1</SUP>H NMR data also suggested the different coordination mode between these two metal complexes in methanol.
- 公益社団法人 日本化学会の論文
著者
-
Hatano Masahiro
Chemical Research Institure Of Non-aqueous Solutions Tohoku University
-
Shimizu Toru
Chemical Research Institute Of Non-aqueous Solutions Tohoku University
-
Kodaka Masato
National Chemical Laboratory for Industry
関連論文
- P-99 SOLID-PHASE SYNTHESIS OF DEHYDROPEPTIDES
- METAL IONS IN CALMODULIN-TRIFLUOPERAZINE SYSTEM : AN APPROACH WITH CD AND NMR METHODS
- SPIN EQUILIBRIUM IN HEMEPROTEIN MODEL
- APPLICATION OF RAPID SCANNING CD SPECTROPOLARIMETER TO BIOMOLECULE ANALYSES
- 57Fe spin-lattice relaxation time of Fe(CO)5 and ferrocene.
- The application of magnetic circular dichroism to the study of the tautomerism of cytosine and isocytosine.
- Magnetic circular dichroism studies of bipyridyls.
- The magnetic circular dichroism of the conjugated O- and S-heterocycles.
- Electronic structures and spectra of a novel diquinone; 1,4:5,8-Anthracenediquinone.
- Magnetic circular dichroism studies on o-, m-, and p-nitroanilines and o-, m-, and p-aminobenzoic acids.
- Tetraoxotetraazaparacyclophanes with various alkyl chains as hosts for hydrophobic compounds.
- Hydrolysis of p-nitrophenyl esters by histidine-containing linear and cyclic peptides.
- Hydrolysis of p-nitrophenyl esters by hydrophobic linear molecule containing two histidine residues.
- CNDO and INDO calculations of the magnetic circular dichroism of several purine dervatives.
- Effects of divalent metal cations on circular dichroism and 1H nuclear magnetic resonance spectra of linear and cyclic peptides having side-chain imidazolyl and acetamido groups.
- Magnetic circular dichroism studies of 4,4'-disubstituted biphenyls.
- Induced Circular Dichroism of Benzyl Chromophores Bound to Helical Polypeptides
- The preparation, infrared spectra, and thermal stability in the solid state of nickel(II) complexes with edda-type polyamino carboxylic acids.
- The conformations of copper(II) complexes with edda-type optically active polyamino carboxylic acids in aqueous solutions.
- The pH-dependent Variation of the Absorption and Circular Dichroism Spectra in Some Ternary Mixed Complexes of Copper(II) Containing Diethylenetriamine and Optically Active Amino Acids
- Circular Dichroism of Copper(II) Complexes with Optically Active Tetradentate Ligands in Aqueous Solutions
- The conformations of nickel(II) complexes with edda-type optically active polyamino carboxylic acids in aqueous solutions.