Effect of pressure on the pre-steady-state kinetics of the hydrolysis of p-nitrophenyl pivalate catalyzed by .ALPHA.-chymotrypsin.
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概要
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The pre-steady-state of the hydrolysis of <I>p</I>-nitrophenyl pivalate (pNPT) catalyzed by α-chymotrypsin (α-CHT) in Tris buffer solutions was measured up to 2.4 k bar at 25 °C. The reaction was initiated by substrate binding, and followed by acylation and deacylation processes. From the pressure dependence on the dissociation constants (<I>K</I><SUB>s</SUB>), the volume change Δ<I>V</I><SUB><I>K</I><SUB>s</SUB></SUB> was −14±1 cm<SUP>3</SUP> mol<SUP>−1</SUP>. From the pressure dependence on the rate constants (<I>k</I><SUB>acyl</SUB> and <I>k</I><SUB>deacyl</SUB>) of the acylation and deacylation processes, the activation volumes Δ<I>V</I><SUB>acyl</SUB><SUP>\neweq</SUP> and Δ<I>V</I><SUB>deacyl</SUB><SUP>\neweq</SUP> were −24±1 and −2±1 cm<SUP>3</SUP> mol<SUP>−1</SUP>, respectively. The reaction mechanisms of α-CHT are discussed in terms of the reaction and activation volumes.
- 公益社団法人 日本化学会の論文
著者
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Suzuki Keizo
Department Of Chemistry Faculty Of Science And Engineering Ritumeikan University
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Taniguchi Yoshihiro
Department Of Applied Chemistry Faculty Of Science And Engineering Ritsumeikan University
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Makimoto Syoichi
Department of Chemistry, Faculty of Science and Engineering, Ritsumeikan University
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