Studies on Taka-Amylase A Under High Pressure : II. Recovery of Enzymic Activity of Pressure Inactivated Taka-Amylase A and its Enhancement by Retreatment at Moderate Pressure
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概要
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The recovery of enzymic actlvity in pressure-inactivated Taka-amylase A and its enhancement by a moderate pressure treatment were examined. The enzyme concentration, pH, temperature, and ionic strength influence the velocity of the recovery of activity to a great extent. The recovery was found to be favored by a high concentration of enzyme, neutral pH, a temperature of about 30°C, and a low ionic strengh. Treatment at a moderate pressure of 600-4000 kg per square centimeter of the high pressure-inactivated Taka-Amylase A enhanced the recovery. The recdvery of the partial]y inactivated Taka-amylase A (compression at 7500 or 6000 kg per square centimeter for 10 minutes) was greater than that of the completely inactivated Taka-Amylase A (compression at 9500 kg per squate centimeter for 10 minutes). Below 1200 kg per square centimeter the extent of the recovery increased as the pressure increased, but above 1200 kg per square centimeter the extent of the recovery gradually decreased. Taka-amylase A inactivated by prolonged compression at 9500 kg per square centimeter at 30° did not recover enzymic activity on standing at atmospheric pressure, but the activity of this inactivated enzyme could be partly recovered by treatment at a moderate pressure. Some kinetic parameters of the recovering reaction were calculated.
- 大阪夕陽丘学園短期大学の論文
- 1965-07-01
著者
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Suzuki Keizo
Department Of Chemistry Faculty Of Science And Engineering Ritumeikan University
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MIYAGAWA KINJIRO
Laboratory of Chemistry, Osaka Joshigakuen College
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SANNOE KIYOTOSHI
Department of Chemistry, Faculty of Science and Engineering, Ritumeikan University
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Miyagawa Kinjiro
Laboratory Of Chemistry Osaka Joshigakuen College
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Sannoe Kiyotoshi
Department Of Chemistry Faculty Of Science And Engineering Ritumeikan University
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