Effect of Unpaired Terminal Nucleotides of Substrate RNAs on the RNA Ligation with T4 RNA Ligase.
スポンサーリンク
概要
- 論文の詳細を見る
The effect of double-helix formations and unpaired terminal nucleotides of RNA oligomer substrates on a RNA ligation with T4 RNA ligase has been investigated by using high-performance liquid chromatography (HPLC), UV absorbance, and circular dichroism (CD) measurements, and the energy calculation for the double-helix stability of RNAs. In the 1-hour reaction of single-stranded RNA acceptors, the yield of the product increased in the order of pyrimidine donors pCp>pUp and purine donors pAp>pGp. In the reaction of a double-stranded RNA acceptor, UGCGCA, the yield also increased in the same order of the donors. On the other hand, in the reaction of double-stranded RNA acceptors containing unpaired terminal nucleotides (dangling ends), pUp and pGp donors gave the largest yields of the products in the reactions of AUGCGCA and CAUGCAU acceptors, respectively. Furthermore, the yield of the product is the largest for CAUGCAU acceptor within the acceptors having A, C, G, and U dangling ends in the reaction of pGp donor. These results sugggest that the formation of the terminal base pairs of the donors and the dangling ends may affect the RNA ligation with T4 RNA ligase.
著者
-
Sugimoto Naoki
Department Of Cardiology Kanazawa Municipal Hospital
-
Sasaki Muneo
Department Of Chemistry Faculty Of Science And Engineering Konan University
-
Matsumura Akiko
Department Of Biotechnology Faculty Oj Engineering Fukuyama University
-
Hasegawa Keiko
Department Of Applied Biological Science Tokyo Noko University
関連論文
- Isolation and Absolute Configuration of SMTP-0, a Simplest Congener of the SMTP Family Nonlysine-analog Plasminogen Modulators
- Inhibitory effect of N-palmitoylphosphatidylethanolamine on macrophage phagocytosis through inhibition of Rac1 and Cdc42
- PJ-672 G_ and G_q Coordinately Mediate S1P_2-induced Inhibition of Rac and Migration in Vascular Smooth Muscle in Rho-dependent Manner(PJ113,Atherosclerosis, Basic 2 (IHD),Poster Session (Japanese),The 73rd Annual Scientific Meeting of The Japanese
- OE-181 Class-IIα Phosphoinositide 3-Kinase is an Essential Regulator of Ca^-Dependent Rho Activation, Myosin Phosphatase and Contraction in Vascular Smooth Muscle Cells(Vascular smooth muscle-1, The 71st Annual Scientific Meeting of the Japanese Circu
- PJ-373 Sphingosine-1-Phosphate Inhibits the Formation of Tube-Like Structures via Its Receptor EdgS in Vascular Endothelial Cells(Angiogenesis 3 (IHD) : PJ62)(Poster Session (Japanese))
- PE-348 G_q- and G_-coupled activation of Rho mediates inhibitory regulation of cellular Rac and chemotaxis in VSMCs(Molecular Biology, Vascular 3 (H) : PE59)(Poster Session (English))
- OE-012 Knockdown of Edg1 Gene Expression by RNA Interference Abolishes Sphingosine-1-Phosphate-Induced Platelet-Derived Growth Factor-B Chain Expression in Vascular Smooth Muscle Cells(Vasoactive Compound/Growth Factor/Cytokine 1 (H) : OE2)(Oral Presentat
- Sphingosine-1-Phosphate Stimulates Gene Expression of the Transcription Factor KLF5/BTEB2, Which Plays a Critical Role in PDGF-B Gene Expression, in VSMCs
- The Small GTPase Rho Mediates Sphingosine-1-Phosphate Induced Inhibition of Chemotaxis Toward PDGF through Inhibiting Rac in Vascular Smooth Muscle Cells
- Roles for the small G proteins and downstream protein kinases in sphingosine-1-phosphate(S1P)-induced PDGF-B chain gene expression in VSMCs