抗Estrogen Receptor (ER) 抗体を用いた酵素抗体法による標的組織内ERの局在

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It is very important to detect the intracellular distribution and dynamics of estrogen receptor (ER) in order to make the mechanisms of estrogen action clear. In a recent report, monoclonal antibodies localized ER only in the nucleus of target cells under both the presence and absence of estrogen. <BR>We purified cow and human uterine ER and induced specific polyclonal anti-ER anti-bodies. In this report, the specificity of immunological reactivity of anti-ER antibodies was examined, and the distribution of ER in the target tissues was detected by the indirect immunoperoxidase method. <BR>The specific staining was observed in the nucleus and cytoplasm of the endo- and myometrium of cow, rat and human uterus. In the nontarget organs, such as kidney and muscle, the specific staining was not detected. The cytoplasm of MCF-7 cells and immature rat uterine tissues were mainly stained without estrogen; however, the nuclear staining in-creased obviously after the estrogen priming. The cytoplasmic and/or nuclear staining was positive in 63% of 21 human breast cancer cases. This immunohistochemical assay (IHC) method of ER measurement was compared with the DCC assay method, a biochemical assay based on hormone binding ability. The concomitant positivity or negativity in DCC and IHC were observed as 61.9% (13 cases) or 19.0% (4 cases), respectively. Therefore, these two methods showed the same assay results in 81.0% of human breast cancer tissues.
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抗Estrogen Receptor (ER) 抗体を用いた酵素抗体法による標的組織内ERの局在

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