成長ホルモンの生物学的検定法

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Tibial assay by measurements of width of epiphyseal plate of hypophysectomized, immature rats of Donryu strain in response to growth hormone and other pituitary or various steroid hormones was studied in regard to sensitivity, precision and specificity with age effects of gonadotropic activity, tibia width and testis weight increments of normal rats. Our investigation deals with 1. The influence of age on the response of the epiphyseal cartilage to growth hormone injections in hypophysectomized rats. 2. The comparison of Long Evans strain with Donryu strain. The rat of Long-Evans strain was approximately 4 times greater in sensitivity than Donryu rats on the increase in thickness of the epiphyseal disc. 3. The importance of a postoperative diet which has great influence on the accuracy of tibia test. The effects of exogenous growth hormone, of course, vary with the dosages used, duration of injection period, and furthermore, the degree of severity of the dietary deficiency concerned. 4. An effort to devise a simple and rapid procedure, practical for routine work. We avoided time-consuming technique such as complex staining methods as described by Greenspan & Evans. Our stain technique requires only 10 minutes. 5. Determination of the accuracy of the method ; standard errors were determined for the experimental groups. The minimal effective dose of a growth hormone preparation was defined as the amount given under the experimental conditions described in this communication which had caused an increase in the width of the epiphyseal plate over the control value of 70 micra. 6. The response of the epiphyseal disc to other hormones, such as pituitary lactogenic hormone, ACTH, insulin, thyroxine and progesterone has been carefully studied. We have observed that lactogenic hormone increased the width of the epiphyseal cartilage plate. This result needs further investigation to confirm our observations, histologically as well.
日本内分泌学会の論文

成長ホルモンの生物学的検定法

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