Screening of Soil-Born Fungi from Forest Soil Using Glyphosate Herbicide as the Sole Source of Phosphorus
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概要
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Glyphosate herbicide was used as screening agent to be the sole source of phosphorus for the isolation of soil-born fungi of forest soil. Three fungal strains were able to grow consistently in the presence of glyphosate as the sole phosphorus source and were identified as <I>Fusarium</I> sp. strain FRP1, <I>Scopulariopsis</I> sp. strain FRP2 and <I>Trichoderma</I> sp. strain FRP3. On standard medium ten fungal strains were isolated and identified as <I>Botrytis</I> sp. strain FR1, <I>Mucor</I> sp. strain FR2, <I>Acremonium</I> sp. strain FR3, <I>Trichoderma</I> sp. strain FR4, <I>Botrytis</I> sp. strain FR5, <I>Crysosporium </I>sp. strain FR6, <I>Scopulariopsis </I>sp. strain FR7, <I>Trichoderma</I> sp. strain FR8, <I>Botrytis</I> sp. strain FR9 and <I>Acremonium</I> sp. strain FR10. Of the three screened fungal species, <I>Scopulariopsis </I>sp. strain FRP2 and <I>Trichoderma</I> sp. strain FRP3 were selected for further study because they had the highest ratio of growth diameter. The growth kinetics of <I>Scopulariopsis</I> sp. strain FRP2 and <I>Trichoderma</I> sp. strain FRP3 were also observed on broth Czapek medium containing glyphosate as the sole source of phosphorus. Synergetic interactions <I>in vitro </I>were examined on PDA. The synergetic interactions of <I>Scopulariopsis</I> sp. strain FRP2 and <I>Trichoderma</I> sp. strain FRP3 as mix culture showed antibiosis inhibition mode.
著者
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Imai Tsuyoshi
Division Of Thermonuclear Fusion Research Japan Atomic Energy Research Institute
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Yamamoto Koichi
Division of Cardiology, Sakurabashi Watanabe Hospital
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HIGUCHI Takaya
Division of Environmental Science and Engineering, Graduate School of Science and Engineering (Engineering), Yamaguchi University
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SEKINE Masahiko
Division of Civil and Environmental Engineering, Graduate School of Science and Engineering, Yamaguchi University, Japan
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HIGUCHI Takaya
Division of Environmental Science and Engineering, Graduate School of Science and Engineering, Yamaguchi University, Japan
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ARFARITA Novi
Faculty of Agrotechnology, Malang Islamic University, Indonesia
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KANNO Ariyo
Division of Civil and Environmental Engineering, Graduate School of Science and Engineering, Yamaguchi University, Japan
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IMAI Tsuyoshi
Division of Environmental Science and Engineering, Yamaguchi University, Japan
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YAMAMOTO Koichi
Division of Civil and Environmental Engineering, Graduate School of Science and Engineering, Yamaguchi University, Japan
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IMAI Tsuyoshi
Division of Environmental Science and Engineering, Graduate School of Science and Engineering, Yamaguchi University, Japan
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