Large-Scale Survey of Mitochondrial D-Loop of the Red-Crowned Crane Grus japonensis in Hokkaido, Japan by Convenient Genotyping Method

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概要

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• The Red-crowned Crane, Grus japonensis, is an endangered species of crane that has two separate breeding populations, one in the Amur River basin (continental population) and the other in eastern or northern Hokkaido, Japan (island population). So far, only two haplotypes (Gj1 and Gj2) have been identified in the mitochondrial D-loop in island population, whereas seven haplotypes have been found in continental population (Gj3–Gj9). We developed a rapid and inexpensive method of extensive genotyping of D-loop haplotypes in Red-crowned Cranes, based on amplification refractory mutation system (ARMS) PCR assay. Two hundred and three cranes in eastern Hokkaido were studied with this method and supplemental DNA sequencing. Only two haplotypes, Gj1 and Gj2, were confirmed in eastern Hokkaido with Gj2 as a major haplotype. Additionally, only Gj2 was identified in twelve feathers from both sexes found in northern Hokkaido. These results suggest scarce genetic diversity in island population of Red-crowned Cranes in Hokkaido, Japan.

著者

• Asakawa Mitsuhiko

  School Of Veterinary Medicine Rakuno Gakuen University

• KITAZAWA Takio

  School of Veterinary Medicine, Rakuno Gakuen University

• HIRAGA Takeo

  School of Veterinary Medicine, Rakuno Gakuen University

• TERAOKA Hiroki

  School of Veterinary Medicine, Rakuno Gakuen University

• MIURA Yoshiaki

  School of Veterinary Medicine, Rakuno Gakuen University, Ebetsu 069–8501, Japan

• SHIRAISHI Junya

  School of Veterinary Medicine, Rakuno Gakuen University, Ebetsu 069–8501, Japan

• SHIOMI Akira

  School of Veterinary Medicine, Rakuno Gakuen University, Ebetsu 069–8501, Japan

• MAKITA Kohei

  School of Veterinary Medicine, Rakuno Gakuen University, Ebetsu 069–8501, Japan

• MOMOSE Yulia

  NPO Tancho Protection Group, Kushiro 085–0036, Japan

• MOMOSE Kunikazu

  NPO Tancho Protection Group, Kushiro 085–0036, Japan

• MASATOMI Hiroyuki

  NPO Tancho Protection Group, Kushiro 085–0036, Japan

• ASAKAWA Mitsuhiko

  School of Veterinary Medicine, Rakuno Gakuen University, Ebetsu 069–8501, Japan

• HIRAGA Takeo

  School of Veterinary Medicine, Rakuno Gakuen University, Ebetsu 069–8501, Japan

• KITAZAWA Takio

  School of Veterinary Medicine, Rakuno Gakuen University, Ebetsu 069–8501, Japan

• TERAOKA Hiroki

  School of Veterinary Medicine, Rakuno Gakuen University, Ebetsu 069–8501, Japan

• MASATOMI Hiroyuki

  NPO Tancho Protection Group

• MOMOSE Kunikazu

  NPO Tancho Protection Group

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