Rhein Inhibits Integrin-Linked Kinase Expression and Regulates Matrix Metalloproteinase-9/Tissue Inhibitor of Metalloproteinase-1 Ratio in High Glucose-Induced Epithelial-Mesenchymal Transition of Renal Tubular Cell

スポンサーリンク

概要

• 論文の詳細を見る

• Studies have found overexpressed integrin-linked kinase (ILK) and disturbed matrix metalloproteinase-9/tissue inhibitor of metalloproteinase-1 (MMP-9/TIMP-1) ratio in diabetic nephropathy epithelial-mesenchymal transition (EMT). However, the underlying mechanisms of EMT and the inhibiting effect of rhein need further understanding. The aim of this study was to investigate the possible regulating effects of ILK towards MMP-9/TIMP-1 ratio in EMT and the inhibiting effect of rhein. The characteristic epithelial marker and mesenchymal marker of EMT were examined by cytoimmunostaining, real-time reverse transcription polymerase chain reaction (real-time RT-PCR) and Western blot. To observe the EMT inhibiting effects of rhein, specific ILK-small interfering RNA (ILK-siRNA) was used as a positive control. The results showed that in high glucose conditions, overexpression of ILK and an abnormal changing of MMP-9/TIMP-1 ratio occurred; ILK inhibition by siRNA could adjust MMP-9/TIMP-1 ratio to near normal. Meanwhile, rhein inhibited the overexpressing ILK and inhibits high glucose-induced EMT; the effect was similar to that of ILK-siRNA. The decreased expression of ILK regulated by rhein contributed to the adjustment of the MMP-9/TIMP-1 ratio. Our data indicates that rhein inhibits high glucose-induced-EMT partially through the inhibition of ILK expression and regulates the MMP-9/TIMP-1 ratio in HK-2 cells. This mechanism may be associated with rhein’s effect of ILK suppression.

著者

• Zhang Jing

  Department Of Anatomy Histology & Embryology School Of Basic Medical Sciences Peking University

• Fu Shuangshuang

  Department of Nephrology, Xiangya Hospital of Central South University

• Peng Linlin

  Department of Nephrology, Xiangya Hospital of Central South University

• Yang Jiayi

  Department of Nephrology, Xiangya Hospital of Central South University

• Ning Chen

  Department of Urology, Xiangya Hospital of Central South University

• Xiao Xiangcheng

  Department of Nephrology, Xiangya Hospital of Central South University

• He Dan

  Department of Nephrology, Xiangya Hospital of Central South University

• Wang Xiaoyuan

  Department of Nephrology, Xiangya Hospital of Central South University

• Li Zhiping

  Department of Nephrology, Xiangya Hospital of Central South University

• Ning Jianping

  Department of Nephrology, Xiangya Hospital of Central South University

関連論文

• Hemin Modulates Cytokine Expressions in Macrophage-Derived Foam Cells via Heme Oxygenase-1 Induction
• Neurotrophic and growth factor gene expression profiling of mouse bone marrow stromal cells induced by ischemic brain extracts
• Endothelin signaling axis activates osteopontin expression through PI3 kinase pathway in A375 melanoma cells
• Ectopic cervical anaplastic ependymoma
• Epigenetic and genetic alterations of PTEN in hepatocellular carcinoma
• Downregulation of MSP58 inhibits growth of human colorectal cancer cells via regulation of the cyclin D1-cyclin-dependent kinase 4-p21 pathway
• Improved Blind Decodings of STBC with Unknown and Known Channel Correlation to Transmitter(Wireless Communication Technologies)
• Stereospecific Radical Polymerization of N-Methyl Methacrylamid
• The Protective Role of Cellular Glutathione Peroxidase Against Trauma-induced Mitochondrial Dysfunction in the Mouse Brain
• Opportunistic Cooperative Communications over Nakagami-m Fading Channels
• Performance Analysis of Opportunistic-Based Two-Way Relaying with Beamforming over Nakagami-m Fading Channels
• P2-74 Morphology investigation on the thyroid from autopsies of patients with Severe Acute Respiratory Syndrome (SARS)
• Protective Effects of Anisodamine on Renal Function in Patients with ST-Segment Elevation Myocardial Infarction Undergoing Primary Percutaneous Coronary Intervention
• An Anti E. Coli O157:H7 Antibody-Immobilized Microcantilever for the Detection of Escherichia Coli (E. coli)
• Productivity-species richness relationship changes from unimodal to positive linear with increasing spatial scale in the Inner Mongolia steppe
• Malignant solitary fibrous tumor arising from the pineal region : case study and literature review
• Rhein Inhibits Integrin-Linked Kinase Expression and Regulates Matrix Metalloproteinase-9/Tissue Inhibitor of Metalloproteinase-1 Ratio in High Glucose-Induced Epithelial-Mesenchymal Transition of Renal Tubular Cell
• Clinical Features of 66 Lymphoma Patients Presenting with a Fever of Unknown Origin
• Distribution of Pu isotopes in marine sediments in the Pacific 30km off Fukushima after the Fukushima Daiichi nuclear power plant accident
• CJK-3 Population pharmacokinetics of piperacillin/tazobactam in neonates and young infants(CJK (China,Japan,Korea) Pharmacist International Symposium)
• Evaluation of natural gas dehydration in supersonic swirling separators applying the Discrete Particle Method
• Intracellular protein cyclization catalyzed by exogenously transduced Streptococcus pyogenes sortase A(ENZYMOLOGY, PROTEIN ENGINEERING, AND ENZYME TECHNOLOGY)
• Oxidative degradation of aqueous phenol effluent with electrogenerated Fenton's reagent.

もっと見る 閉じる

スポンサーリンク