Laminin Isoforms and Laminin-Producing Cells in Rat Anterior Pituitary
スポンサーリンク
概要
- 論文の詳細を見る
Laminin is a key component of the basement membrane and is involved in the structural scaffold and in cell proliferation and differentiation. Research has identified 19 laminin isoforms, which are assemblies of α, β, and γ chains (eg, the α1, β1, and γ1 chains form the laminin 111 isoform). Although laminin is known to be present in the anterior pituitary, the specific laminin isoforms have not been identified. This study used molecular biological and histochemical techniques—namely, RT-PCR, immunohistochemistry, and in situ hybridization—to identify the laminin isoforms and laminin-producing cells in rat anterior pituitary. RT-PCR showed that laminin α1, α3, and α4 genes were expressed in anterior pituitary. Immunohistochemistry revealed laminin α1 in gonadotrophs and laminin α4 in almost all vascular endothelial cells. Laminin α3 was seen in a subset of vascular endothelial cells. We then performed in situ hybridization to localize β and γ chains in these cells and found that laminin β1, β2, and γ1 were expressed in gonadotrophs and that laminin β1 and γ1 were expressed in endothelial cells. In conclusion, we identified gonadotroph-type (laminin 111 and 121) and vascular-type (laminin 411 and 311) laminin isoforms in rat anterior pituitary.
著者
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Fujiwara Ken
Division Of Histology And Cell Biology Department Of Anatomy Jichi Medical University School Of Medi
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Horiguchi Kotaro
Division Of Histology And Cell Biology Department Of Anatomy Jichi Medical University School Of Medi
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Kikuchi Motoshi
Division Of Histology And Cell Biology Department Of Anatomy Jichi Medical School
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Yashiro Takashi
Division Of Histology And Cell Biology Department Of Anatomy Jichi Medical School
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HORIGUCHI Kotaro
Division of Histology and Cell Biology, Department of Anatomy, Jichi Medical University School of Medicine
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Tsukada Takehiro
Division of Histology and Cell Biology, Department of Anatomy, Jichi Medical University School of Medicine
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Yashiro Takashi
Division of Histology and Cell Biology, Department of Anatomy, Jichi Medical University School of Medicine
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Ramadhani Dini
Division of Histology and Cell Biology, Department of Anatomy, Jichi Medical University School of Medicine
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Fujiwara Ken
Division of Cell Biology and Histology, Department of Anatomy, Jichi Medical University School of Medicine
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Yashiro Takashi
Division of Cell Biology and Histology, Department of Anatomy, Jichi Medical University School of Medicine
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