Effect of E-cadherin Expression on Hormone Production in Rat Anterior Pituitary Lactotrophs In Vitro
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概要
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Cadherins are a family of transmembrane glycoproteins that mediate cell-to-cell adhesion. A change in cadherin type in cells, i.e., cadherin switching, induces changes in the character of the cell. Recent studies of the developing rat adenohypophysis found that primordial cells co-expressed E- and N-cadherins, but that hormone-producing cells lost E-cadherin and ultimately possessed only N-cadherin. In the present study, we examined the roles of cadherin switching in cytogenesis of anterior pituitary cells by observing prolactin mRNA and protein expression in lactotrophs that were transformed with an E-cadherin expression vector. In hormone-producing cells that were transfected with a pIRES2-ZsGreen1 plasmid with a full-length E-cadherin cDNA (rE-cad-IZ) insert in primary culture, we detected E- and N-cadherins on plasma membrane and E-cadherin in cytoplasm. In these rE-cad-IZ-transfected cells, in situ hybridization revealed prolactin mRNA signals that were at a level identical to that in control cells, while prolactin protein was barely detectable using immunocytochemistry. The mean signal intensity of prolactin protein in rE-cad-IZ-transfected cells was approximately one fourth that in intact cells and in null-IZ-transfected cells (P<0.01). These results suggest that the expression of E-cadherin does not affect prolactin mRNA transcription; rather, it reduces prolactin protein content, presumably by affecting trafficking of secretory granules.
著者
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Kouki Tom
Division Of Histology And Cell Biology Department Of Anatomy Jichi Medical University School Of Medi
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Kusumoto Kenji
Division Of Histology And Cell Biology Department Of Anatomy Jichi Medical University School Of Medi
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Horiguchi Kotaro
Division Of Histology And Cell Biology Department Of Anatomy Jichi Medical University School Of Medi
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Kawanishi Kotaro
Division Of Histology And Cell Biology Department Of Anatomy Jichi Medical University School Of Medi
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Kikuchi Motoshi
Division Of Histology And Cell Biology Department Of Anatomy Jichi Medical School
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KOUKI Tom
Division of Histology and Cell Biology, Department of Anatomy, Jichi Medical University School of Medicine
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Fujiwara Ken
Division of Cell Biology and Histology, Department of Anatomy, Jichi Medical University School of Medicine
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Yashiro Takashi
Division of Cell Biology and Histology, Department of Anatomy, Jichi Medical University School of Medicine
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