肝疾患とCeruloplasmin代謝に関する研究

概要

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Ceruloplasmin, a copper protein in the human serum, was purified with DEAE-cellulose column chromatography and alcohol-chloroform. The purified ceruloplasmin was labelled with radioactive iodine according to the method of Hunter. With the preliminary experiments the purified ceruloplasmin was pure in electrophoretic analysis. And it was also confirmed that 125I-labelled ceruloplasmin did not degenerate with radiation of labelled iodine itself and iodine of 125I-labelled ceruloplasmin did not move to the other protein moiety in the serum in vitro and vivo. Labelled ceruloplasmin was injected intravenously to the patients of liver disease and controls. Blood samples were drawn at 5 and 30, 60 min and 3, 6 and 12 hours after the injection and thereafter, daily untill the fourteenth day. Analysing the serum radioactivity disappearance curve, the biological half life of the injected ceruloplasmin was much longer in patients of livercirrhosis. The radioactivity found in urine, bile and feces, was seemed to be excreted as a component of a small molecular substances. It was assumed that the rate of uptake and degradation of the ceruloplasmin in the body also contributed as one of factors of controlling serum ceruloplasmin level other than those of synthetic ability in the liver.
学校法人昭和大学・昭和医学会の論文