Studies on prostaglandin synthetic activities in mouse salivary glands.
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Prostaglandin (PG) synthetic activities in mouse salivary glands were studied. When mouse submandibular gland homogenate was incubated with radioactive arachidonic acid, three radioactive metabolites were detected which were identified with PGE<SUB>2</SUB>, PGF<SUB>2α</SUB>, and PGI<SUB>2</SUB>. Thromboxane A<SUB>2</SUB> (TXA<SUB>2</SUB>) and PGD<SUB>2</SUB> were not detected. Optimal biosynthesis was obtained in an 0.05M sodium phosphate buffer at pH7.4. Under this condition PG formation was linear up to the arachidonic acid concentration of 40μM. Reduced glutathion did not affect the biosynthesis, but catecholamines i.e. L-adrenaline, L-noradrenaline and L-isoproterenol produced only a small stimulation of all three PG synthesis in the submandibular gland homogenate. Major cyclooxygenase products of the parotid gland and the sublingual gland were also PGE<SUB>2</SUB>, PGF<SUB>2α</SUB>, and PGI<SUB>2</SUB>. Total PG synthetic activities of three PG<SUB>s</SUB> in both the submandibular gland and the parotid gland were higher than those in the pancreas, liver and brain, and that of the submandibular gland was the highest of the examined tissues. Both PGE<SUB>2</SUB> and PGI<SUB>2</SUB> synthetic activities were higher in the order of submandibular gland>parotid gland>sublingual gland. PGF<SUB>2α</SUB> synthetic activity was higher in the order of parotid gland>submandibular gland>sublingual gland PGI<SUB>2</SUB> synthetic activity in the submandibular gland of male mouse was about 1.5 times higher than that of female one.<BR>Next, the relationship between PG metabolism and DNA synthesis in isoproterenol (IPR)-stimulated salivary glands was investigated. Administration of indomethacin, a PG synthesis inhibitor, suppressed the effect of IPR on the DNA synthesis in the submandibular gland and the parotid gland. Cyclooxygenase activity did not change in IPR-stimulated salivary glands. From these results, it is suggested that PG<SUB>s</SUB> are involved in the stimulation of the DNA synthesis by IPR in the salivary glands. It is also possible that IPR activates not a cyclooxygenase, but a phospholipase, in the salivary glands.
- 社団法人 日本口腔外科学会の論文
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