ENZYMATIC SYNTHESIS OF THIAMINE:II. THE THIAMINE SYNTHESIS FROM PYRIMIDINE AND THIAZOLE PHOSPHATES AND THE ENZYMATIC SYNTHESIS OF PYRIMIDINE MONO- AND DIPHOSPHATE AND THIAZOLE MONOPHOSPHATE

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概要

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• 1. Phosphates of 2-methyl-4-amino-5-hydroxymethylpyrimidine (OMP) and 4-methyl-5-β-hydroxymethyl thiazole (Th) were synthesized chemically and the activities of thiamine synthesis without ATP were examined. OMP pyrophosphate (OMP-PP) and Th phosphate (Th-P) were most active as substrates and had a reaction rate of 5 times as much as that of the thiamine synthesis from OMP and Th with ATP. OMP-PP and Th pyrophosphate (Th-PP) had also a fair activity as substrates.2. The thiamine synthesized from OMP-PP and Th-P was proved to be a mixture of thiamine and thiamine monophosphate, while that from OMP-PP and Th-PP a mixture of thiamine, thiamine monophosphate and thiamine pyrophosphate.3. The active compound of OMP was isolated by Dowex 1 column chromatography from the enzyme reaction mixture incubated with OMP-P32 or ATP-γ-P32. The isolated OMP-PP formed from OMP-P32 and ATP, and OMP-P and OMP-PP formed from OMP and ATP-γ-P32 were identified by using paper chromatography, bioautography with a E. coli mutant, actigraphy and absorption spectra.4. Comparing the specific radioactivities of the OMP-P and OMP-PP isolated with those of OMP-P32 or ATP-γ-P32 added, OMP-PP is presumed to be mainly formed by successive phosphorylation of the γ-phosphate of ATP through OMP-P.5. The active intermediate of Th enzymatically formed was identified bioautographically as Th-P and the mechanism of enzymatic synthesis of thiamine was discussed.

• 日本ビタミン学会の論文

著者

• IWASHIMA AKIO

  Biochemical Institute, Kyoto Prefectural University of Medicine

• NOSE YOSHITSUGU

  Biochemical Institute, Kyoto Prefectural University of Medicine

• UEDA KIYOSHI

  Biochemical Institute, Kyoto Prefectural University of Medicine

• KAWASAKI TAKASHI

  Biochemical Institute, Kyoto Prefectural University of Medicine

• FUJITA TETSURO

  Biochemical Institute, Kyoto Prefectural University of Medicine

関連論文

• ENZYMATIC SYNTHESIS OF THIAMINE:II. THE THIAMINE SYNTHESIS FROM PYRIMIDINE AND THIAZOLE PHOSPHATES AND THE ENZYMATIC SYNTHESIS OF PYRIMIDINE MONO- AND DIPHOSPHATE AND THIAZOLE MONOPHOSPHATE
• ENZYMATIC SYNTHESIS OF THIAMINE:I. THE ENZYMATIC SYNTHESIS OF THIAMINE FROM PYRIMIDINE AND THIAZOLE MOIETIES BY A CELL-FREE EXTRACT OF YEAST
• ISOLATION OF THE THIAMINE DEGRADATION PRODUCTS FORMED BY SHELLFISH THIAMINASE
• THE ISOLATION OF ANILINOTHIAMINE FORMED FROM THIAMINE AND ANILINE BY BACILLUS ANEURINOLYTICUS KIMURA ET AOYAMA
• THE SECOND TYPE OF BACTERIAL THIAMINASE

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