ENZYMATIC SYNTHESIS OF THIAMINE:I. THE ENZYMATIC SYNTHESIS OF THIAMINE FROM PYRIMIDINE AND THIAZOLE MOIETIES BY A CELL-FREE EXTRACT OF YEAST

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概要

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• The enzymes obtained from a cell-free extract of bakers' yeast catalyzed the condensation of the pyrimidine (OMP) and thiazole (Th) moieties of thiamine to form thiamine. ATP and Mg2+ were required essentially for the reaction and Mn2+ also showed a fair activity. An optimal pH of the reaction was 7.0. Some other pyrimidine compounds which differ from OMP in the chemical structure at the C5-position of pyrimidine are less active than OMP as the substrates. The enzymatic activations of OMP and Th by ATP in a preliminary step before the condensation were demonstrated.

• 日本ビタミン学会の論文

著者

• NOSE YOSHITSUGU

  Biochemical Institute, Kyoto Prefectural University of Medicine

• UEDA KIYOSHI

  Biochemical Institute, Kyoto Prefectural University of Medicine

• KAWASAKI TAKASHI

  Biochemical Institute, Kyoto Prefectural University of Medicine

関連論文

• ENZYMATIC SYNTHESIS OF THIAMINE:II. THE THIAMINE SYNTHESIS FROM PYRIMIDINE AND THIAZOLE PHOSPHATES AND THE ENZYMATIC SYNTHESIS OF PYRIMIDINE MONO- AND DIPHOSPHATE AND THIAZOLE MONOPHOSPHATE
• ENZYMATIC SYNTHESIS OF THIAMINE:I. THE ENZYMATIC SYNTHESIS OF THIAMINE FROM PYRIMIDINE AND THIAZOLE MOIETIES BY A CELL-FREE EXTRACT OF YEAST
• ISOLATION OF THE THIAMINE DEGRADATION PRODUCTS FORMED BY SHELLFISH THIAMINASE
• THE ISOLATION OF ANILINOTHIAMINE FORMED FROM THIAMINE AND ANILINE BY BACILLUS ANEURINOLYTICUS KIMURA ET AOYAMA
• THE SECOND TYPE OF BACTERIAL THIAMINASE

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