Changes in the ATP Content of Microbial Cells During Heat Treatment
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概要
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To examine whether ATP in microbial cells could survive a thermal treatment, changes in microbial ATP content during the heat treatment were studied with a bioluminescent assay. The ATP content in<I>Escherichia coil</I>cell lysate did not decrease when heated at 90°C for up to 10 min. When cell suspensions of<I>E. coli, Klebsiella</I>sp., <I>Staphylococcus aureus</I>, and<I>Saccharomyces cerevisiae</I>were heated at 58°C, the content of the whole cell suspension ATP, ATP<SUB>w</SUB>, slightly decreased to a certain level. The viable cells of the suspensions measured by a plate count method were rapidly inactivated. Changes in the contents of ATP<SUB>W</SUB>, ATP<SUB>c</SUB> (cell fraction), and ATP<SUB>f</SUB> (cell filtrate fraction) of microbial cell suspensions during the heating process at 58°C were then studied. The contents of ATP<SUB>w</SUB> and ATP<SUB>c</SUB> gradually decreased for all organisms. The increase in the contents of ATP<SUB>f</SUB> for<I>E. coli</I>and<I>Klebsiella</I>sp. showed that the intracellular ATP would leak out. When<I>E. coil</I>cells were heated at various temperatures of 66-54°C, the content of ATP<SUB>w</SUB> decreased to a similar level at these temperatures. The contents of ATP<SUB>c</SUB> and ATP<SUB>f</SUB> approached to certain levels, which were temperature dependent.
- 日本防菌防黴学会の論文
- 2002-03-20
著者
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Fujikawa Hiroshi
Department Of Microbiology Tokyo Metropolitan Institute Of Public Health
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Fujikawa Hiroshi
Department Of Microbiology Tokyo Metropolitan Research Laboratory Of Public Health
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Morozumi Satoshi
Department Of Microbiology Tokyo Metropolitan Institute Of Public Health
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FUJIKAWA HIROSHI
Department of Applied Veterinary Science, the Graduated School of Veterinary Sciences, Gifu University
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