An acid hematein staining method suitable for detecting drug-induced lipidosis.
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概要
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Drug-induced lipidosis is characterized ultrastructurally by the occurrence of myeloid bodies, which are probably due to the formation of drug-lipid complexes. Histochemical acid hematein tests are currently applied to detect lipidosis. In order to clarify the differences in these stainings, three acid hematein techniques were compared ; Baker's original method, a modified method of AFIP, and a simplified method devised by Hori. Liver tissues were obtained from rat treated orally with 4, 4'-Diethylaminoethoxy-hexestrol. The procedures of dichromate treatment are critically different between Baker's and the other two methods. Dark blue stained granules were clearly observable in the cytoplasm of hepatocytes and Kupffer cells with Baker's method. The appearances of staining were confirmed by the results of electron microscopic examination. Positive reactions were eliminated by a pyridine extraction test. The other two methods yielded only diffused dark blue reactants. These results indicate that dichromate treatment before the freezing of the samples as done in Baker's method may be an important process for the simultaneous oxidation, chromation, and fixation of lipids, particularly for demonstrating the presence of myeloid bodies. We also examined the effects of fixation periods with 20% neutral buffered formalin in Baker's method. The reaction products were still detectable even after six months, although the color intensity was weaker.
- 日本毒性病理学会の論文
日本毒性病理学会 | 論文
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