GENETICAL SUSCEPTIBILITY OF RATS TO KIDNEY TUMOR INDUCED BY N-METHYL-N-NITROSOUREA
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N-methyl-N-nitrosourea (MNU) 50mg/kg b.w. was administered to 50-day-old LE, WFN and F1A (WFN×LE) rats maintained in our laboratory and after 24 weeks of MNU treatment they were sacrificed and served for pathological study. The developed tumors were mainly kidney, mammary tumors, and leukemia. Kidney tumor was histologically considered to be of mesenchymal origin similating a fibrosarcoma, and mammary tumors were tubular, papillary or compact type adenocarcinomas and fibromas. The mesenchymal tumors of kidney was observed only in WFN rats and its incidence was as high as 46.7%. DNA synthesis of the kidney at 50 days was examined. Bromodeoxyuridine (BrDU) 20mg/200g b.w. was injected into peritoneal cavity in LE, WFN, F1A rats and after 60min they were sacrificed and incorporation of BrDU in the kidney cells was examined by anti-BrDU monoclonal antibody and ABC method. The mean incorporated cell numbers in one kidney by 10 fields were LE 4.3±3.4, WFN 16.3±7.3, F1A 2.5±2.6. BrDU uptake in WFN rat kidney was significantly (P<0.01) elevated. This finding well correlated with the occurrence of kidney tumor.
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日本毒性病理学会 | 論文
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