四塩化炭素投与によるハマチの血液および肝すい臓成分の変化
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Two series of experiments were performed. In Exp.-1 yellow tail were injected with carbon tetrachloride at the dose of 0.05, 0.1, and 0.2ml/100g B. W., respectively. After 48 hours the fish were sacrificed for chemical analysis and histological observa-tions. In Exp.-2 the fish were administered at dose levels of 0.05 and 0.1ml/100g B. W., and after 0 (non-treated), 2, 6, 24 and 48 hours they were treated similarly as in Exp.-1. Separation of cell cords, congestion of sinusoid and decrease in the number and size of oil droplets in hepatopancreas were observed in fish injected at the dose of 0.1ml/100g B. W. in Exp.-1. Contrary to mammals, lipids in the hepatopancreas showed a tendency to decrease on administration of the chemical. Activities of lactic dehydrogenase (LDH), glutamic oxaloacetic transaminase (GOT) and glutamic pyruvic transaminase (GPT) in serum increased markedly immediately after injection of the chemical. Serum triglyceride, total cholesterol, β-lipoprotein and protein decreased after 2 to 6 hours, but recovered to control levels 24 to 48 hours later. Serum protein of the control was separated into 5 fractions by electrophoresis on a cellulose acetate membrane. However, in yellow tail treated with the chemical at the dose of 0.1ml/100g B. W., a decrease of the A/G ratio was observed, and moreover in fish injected at the dose of 0.2ml/100g B. W., new fragments appeared between II and III, and the IV and V fractions.
- 日本水産學會の論文
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