ハマチのビタミン要求に関する研究-1-B6およびC欠乏症について

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Although vitamin requirements by fresh water fish have been studied extensively by many workers, only few works have been reported on marine fishes. We studied vitamin B6 or C deficiency syndromes of the yellowtail Seriola quinqueradiata TEMMINCK et SCHLEGEL by feeding the fish with diets composed of white fish meal, gluten, glucose, cod liver oil, vitamin-mixture without vitamin B6 or C, and mineral-mixture. Both vitamin B6 and C deficiency symptoms appeared after about 40 days (Fig. 1 and Table 2). Characteristics of B6 avitaminosis of the yellowtail were anorexia, retarded growth, nervous disorder, high mortality, injury at mouth and rostrum regions, exophthalmia and scoliosis (Table 2 and Figs. 1 and 2). The fish fed with a died lacking vitamin C showed loss of activity and appetite, retarded growth, change of body color, poor growth of operculum, scoliosis, amputation of strait, and hemorrhage in the laterodorsal region (Table 2 and Figs. 1 and 3). Fat content in the liver decreased in both B6 and C deficient fish (Table 3). Serum protein of the yellowtail was separated into four fractions by electrophoresis on cellulose acetate membrane (Fig. 5). In the serum of the fish fed with vitamin B6 or C deficient diet fraction I decreased but fractions III and IV increased (Table 4).
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