プロスタグランディンによるキンギョCarassius auratusのin vitro排卵誘起効果

概要

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The in vitro effects of various prostaglandins (PGE1, PGE2, PGF1α and PGF2α) on ovulation were investigated in isolated goldfish Carassius auratus oocytes previously matured in vivo by injection of human chorionic gonadotropin. PGE1, PGE2, PGF1α and PGF2α all induced ovulation; PGF group was more effective than PGE group, PGF 2αbeing the most effective. Over 50% of oocytes underwent ovulation 15 to 30min after the beginning of incubation with PGF2αat a concentration of 1μg/ml; over 80% of oocytes ovulated within 1 hr. Observations of the ovulatory process in vitro were made using both dissecting microscope and scanning electron microscope. Oocytes from ovarian fragments were expelled into the incubation medium through a rupture in the follicle at the point corresponding to attachment to the overlying epithelium. In situ, the rupture at this point would allow the oocyte to be expelled directly into the ovarian lumen. Ovulation may be cause by contraction of the ovarian follicle layer.
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