ニジマスに浸漬投与された牛血清アルブミン(BSA)の血中動態に及ぼす水温の影響〔英文〕

概要

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To clarify the effects of environmental water temperature on antigen kinetics in the plasma after hyperosmotic infiltration (HI) treatment, bovine serum albumin (BSA) was administered to rainbow trout Oncorhynchus mykiss by HI treatment or by intravenous injection at various water temperatures (9.0, 12.0, 15.0, 17.5, and 20°C). BSA in the plasma was determined by Laurell-rocket immunoelectrophoresis. First, using a least squares program, the BSA clearance rate from the plasma was computed from the plasma BSA levels of the fish injected with BSA at each temper-ature. Then, using this clearance rate and the plasma BSA level after the HI treatment, the de-convolution method was applied to estimate the BSA release rate into the plasma after the HI treatment. The results showed that both the BSA clearance rate and the gross BSA release were significantly correlated to the environmental temperature between 9.0 and 20.0°C (P<0.001). It is suggested that low environmental temperatures interfere with bath immunization.
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