ヒオウギガイ受精卵の前核形成過程に及ぼす減数分裂時の水圧処理およびサイトカラシンB処理の影響〔英文〕

概要

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To examine the effect of cytochalasin B(CB) and hydrostatic pressure treatments on zygotes in Chlamys nobilis for induction of triploidy, we compared the pronuclear events in pressure-treated and CB-treated zygotes at the second meiosis by fluorescence microscopy. The second polar body formation of zygotes was inhibited by treating with CB or hydropressure. In CB-treated zygotes chromosomes were segregated at the second meiosis and developed to two female pronuclei. On the other hand, in pressure-treated zygotes segregation of chromosomes was inhibited and one large diploid female pronucleus was formed. These results suggest CB inhibited only cytokinesis (polar body formation) but pressure inhibited both karyokinesis (segre-gation of chromosomes) and cytokinesis. Although pronuclear events in pressure- and CB-treated zygotes were different, chromatin of pronuclei was condensed normally and became the metaphase chromosome for the first cleavage. These zygotes were thought to deveolp into triploids.