カワマスにおける酵素の電気泳動並びに熱安定性変異支配遺伝子座と動原体間組換え率の推定〔英文〕

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Application of chromosome set manipulation to the pacific abalone Haliotis discus hannai has made it possible to induce triploid sib populations, to estimate rate of gene-centromere recombination at some isozyme loci, and to suggest differential contribution of oocytes by maternal genotypes of thermostability variations of enzymes at multiple loci in the species.The present paper describes successful induction of triploid and gynogenetic diploid animals in the brook trout Salvelinus fontinalis and its hybrid with the japanese char S. leucomaenis after treating inseminated eggs by heat or hydrostatic pressure shock. Analyses on isozyme genotypic relations between parents and their triploid and/or gynogenetic diploid offsprings resulted in to estimate rate of gene-centromere recombination at the five loci. Apparent association between the differential contribution and their parental genotypes of thermostability variation was observed, supporting the results disclosed earlier in the pacific abalone. In addition, evidence of excessive occurrence of heterozygote or deficiency of homozygote was seen in the normal diploid offspring population at alcohol dehydrogenase and superoxide dismutase loci.