紫外線照射によるサケ目魚類4種の雌性発生誘起

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UV irradiation methods for inducing gynogenesis in Salmoniformes are described. Milt was diluted with seminal plasma of chum salmon in a volume rate of about 1:100, and rradiated with a sterilizing lamp. In order to spread the diluted milt throungout the bottom of plastic Petri dish to a depth of 0.1mm, the hydrophobic nature of the suface was turned hydrophile using an ion spattering equipment. When milt was irradiated with doses varying from 80 to 14, 400 ergs/mm2 at the rate of 40 ergs/mm2/sec, the survival rates of embryos which had been inseminated with irradiated milt, showed a typical "Hertwig effect" in four species used in the present study, Osmerus eperlanus mordax, Salvelinus leucomaenis, Salmo gairdneri and Oncorhynchus masou. Fertilization rates were not affected by irradiation at doses ranging from 40 to 4, 800 ergs/mm2 but sperms lost their fertilizing ability completely at a dosage of 14, 000 ergs/mm2. The most harmful dosage was 600 ergs/mm2, at which the survival rates were only 7.3 per cent in O. eperlanus mordax (3 days after insemination), 42.9 per cent in S. leucomaenis (29 days), 2.6 per cent in S. gairdneri (18 days) and 40.1 per cent in O. masou (25 days), while the survival rates of unirradiated groups were more than 90 per cent the same number of days after insemination respectively. Better survival rates were observed at higher does. All embryos showed haploid syndromes at does more than 2, 400 ergs/mm2 and their haploidy was confirmed by chromosome observations. It was elucidated that the inducing rates of gynogenesis using ultraviolet rays were comparable to those in irradiation of ioizing rays.
日本水産學會の論文

紫外線照射によるサケ目魚類4種の雌性発生誘起

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