ツベルクリンアレルギーに関する実験的研究 : 白血球遊走阻止因子 (LIF) とマクロファージ遊走阻止因子 (MIF) の免疫学的意義について
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Both the macrophage migration inhibitory factor (MIF) and leucocyte migration inhibitory factor (LIF) in guinea pigs vaccinated (V) or vaccinated-challenged (VC) with heat-killed BCG were studied in relation to delayed type hypersensitivity, and the results were compared with that in normal guinea pigs (N).<BR>Macrophage inhibitory capacity was determined by direct and indirect migration inhibition tests by J. R. David. Leucocyte inhibitory capacity was determined by direct and indirect migration inhibition tests by M. Søborg.<BR>1) Three kinds of PPD concentrations (20, 40, 80μg/m<I>l</I>) were used in the culture media of lymphocytes obtained from sensitized animals (Fig. 2). The migration indices of MIF and LIF were determined at 24 hours (Figs 3 and 4). These conditions were established on the results of preliminary experiments.<BR>2) The results of both the direct macrophage migration inhibition test and the direct leucocyte migration inhibition test showed remarkable migration inhibitory capacity not only in the V-group but also in the VC-group (Table 1, and Fig. 5).<BR>3) MIF and LIF production of peripheral blood lymphocytes were tested by using the indirect migration inhibition test. Both productions were remarkable only in the V-group, and MIF productions were weak in the VC-group. LIF productions in the VC-group were not demon strable, because migration promoting factors accelerated the migration of target cells in the test involved (Table 2, and Fig. 6).<BR>4) MIF and LIF productions were determined in the spleen cells and the inguinal lymphnode cells of the V-and VC-groups, and compared with the V-and VC-groups of blood lympho-cytes by using the indirect migration inhibition test. The results showed that remarkable MIF productions were seen in the lymph node cells of the V-group as well as in the peripheral blood lymphocytes. On the contrary, the spleen cells of the V-group did not produce MIF at all, although remarkable MIF productions were seen in the spleen cells of the VC-group. On the other hand, MIF productions of VC-lymphnode cells were as weak as the production of the VC-peripheral blood lymphocytes (Fig. 7). LIF production of spleen cells and inguinal lymph node cells were remarkable in the V-group as well as in the peripheral blood lymphocytes. However, LIF productions of all those cells in the VC-group were not shown, because the leucocyte migration was accelerated by unknown factors as in the results seen in VC-peripheral blood lymphocytes (Fig. 8).<BR>5) Peripheral blood lymphocytes of the V-group incubated with anti-thymocyte serum reduced the productions of MIF and LIF. In addition, peripheral blood lymphocytes of the VC-group incubated with anti-thymocyte serum lost not only MIF producing capacity but also the producing capacity of migration promoting factors (Figs 9, and 10). These results suggest that MIF and LIF are mainly produced by T-lymphocytes and that the migration promoting factors are also produced by T-lymphocytes.<BR>It was demonstrated that LIF plays an important role in delayed type hypersensitivity as does MIF. However, immunological behaviors of both lymphokines were somewhat different. This difference must be investigated in a future study concerning delayed type hypersensitivity.
- 日本結核病学会の論文
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- ツベルクリンアレルギーに関する実験的研究 : 白血球遊走阻止因子 (LIF) とマクロファージ遊走阻止因子 (MIF) の免疫学的意義について