結核菌のマイコバクテリオシソ型別
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Mycobacteriocin produced by human type tubercle bacilli could be demonstrated easily on eggmedium containing 0.05% tween 80 by means of stab culture or streak plate methods, and elevenmycobacteriocin-types have been recognized on the basis of the inhibition pattern by using 9indicator strains of rapidly growing mycobacteria.<BR>At present, types 1 (4.9%), 2 (13%), 4 (38.5%), 9 (2%) and 11 (14.8%) showed a stablemycobacteriocin pattern, but types 3 (7.8%) and 7 (6.7%) were apt to show type replacement totypes 2 and 4, respectively, and strains belonging to types 5, 6 and 8 were few, less than 1.5%to the total. In order to perform a reproducible mycobacteriocin typing, our previous typingscheme was revised to be consisted of five groups (A-E). Among 438 strains, mycobacteriocingroup C containing types 4 and 7 was 46.1% of strains tested, group B containing types 2, 3 and6 was 22.6%, group E containing type 11 was 15.1%, group A containing types 1 and 8 was5.5%, group D containing types 9 and 10 was 4.1%, and untypable strains were no more than 6.6% among all tested strains.<BR>Human type tubercle bacilli classified as type 4, which consisted of more than 45% of strainstested, could be divided into three sub-types from sensitivity to M. gordonae 1324 and M. terrae 1450.<BR>Mycobacteriocin producing strains belonging to type D: 9 showed an extremly wide antibacterialactivity to strains of more than 90% of rapidly growing mycobacteria (42 strains), to about 40% of slow growing mycobacteria (29 strains), and even to 10 strains of <I>Staphylococcus aureus</I>.
- 日本結核病学会の論文
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