Stability of Alpha-Ethylthioisonicotinamide (1314 Th)-Resistance in Tubercle Bacilli Occurring in Tuberculous Patients.

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Tuberculous patients having chronic cavities and excreting tubercle bacilli resistant to streptomycin, isoniazid and PAS were treated with 1314 Th (daily 0.5 gm) for four months and it was observed whether or not 1314 Th-resistant population of tubercle bacilli produced by the treatment is persistent after the cease of 1314 Th administration. Drug resistance test was made by the so-called "actual count method". This method consists of the determination of drug resistance level as the highest concentration of drug, on which small inocula consisting of 10 to 150 viable cell units (nearest to 50)can grow as visible colonies after 6 weeks of incubation at 37°C. Medium used was Ogawa's egg medium consisting of the following composition: basal sulution (1 % KH2PO4 and 1 % sodium glutamate), 100 ml; whole eggs, 200 ml; glycerol, 6 ml; 2 % malachite green solution, 6 ml (the resulting pH 6.8). The drug was dissolved first in propylene glycol and then diluted with distilled water. The drug solution was added to the medium before sterilization to obtain a series of the following final concentrations; 0, 5, 10, 20, 30, 50, 100 and 200μg/ml. The limit of 1314 Th resistance was defined as a level of 30 μg/ml resistance in view of the results obtained previously. The observation was conducted during 5 to 17 months after the cease of 1314 Th treatment. Resistances to streptomycin, isoniazid, PAS and kanamycin also were measured by the actual count method. Viable numbers of tubercle bacilli excreted per day were measured in every determination of drug resistance.
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Stability of Alpha-Ethylthioisonicotinamide (1314 Th)-Resistance in Tubercle Bacilli Occurring in Tuberculous Patients.

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