Endo-N-acetylmuramidaseによるLactobacillus caseiプロトプラストの調製

概要

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Lactobacillus casei ATCC 7469, refractory to egg white lysozyme, was successfully converted to protoplast by treating with endo-N-acetylmuramidase (M-1 enzyme) purified from the culture supernatant of Streptomyces globisporus. The activity of M-1 enzyme was enhanced by the addition of a urtain disaccharide, e.q. sucrose or lactose as an osmoregulator for protoplast formation. The protoplast prepared by treating with M-1 enzyme under 0.9M sucrose for 10min retained the ability of amino acid transport as high as that of the intact cell. Thus, these protoplasts may be useful for biochemical and genetical studies of Lactobacillus.
日本細菌学会の論文