Lactobacillus salivariusのテンペレートファージPLS-1による形質導入

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A temperate phage designated as PLS-1 was studied on transducibility and lysogenization. It had been derived from the lysogenic SaS strain of Lactobacillus salivarius. This paper deals for the first time with the transduction in Lactobacilli.Survival or lysogenized fractions were affected remarkably by phage-adsorption medium and temperature. For instance, the survival fractions of PLS-1-adsorbed cells in International Lactobacillus Subcommittee (ILS) medium at 37C were 7, 9, and 20% at a multiplicity of infection 10, 5, and 1, respectively. These fractions increased to 33, 41, and 67% in correspondence with the multiplicity of infection mentioned above, by changing the adsorption temperature to 30C and the adsorption medium ILS to yeast peptone broth. This change resulted in an increase in fractions of lysogenized colonies in survivors from 25 to 50% at a multiplicity of infection 10. In any of the conditions examined, nonlysogenic and phage-PLS-1-sensitive colonies appeared in the survivors at a high frequency.This phage carried out a generalized transduction in L. salivarius. Of all the auxotrophic mutants, those requiring lysine, prolin, or serine were transduced to prototrophy at a frequency of 10-7 to 10-8. A lactose-non-fermenting mutant was also transduced to a fermentable wild type at a similar frequency. Most of the transductants were proved to be sensitive to phage PLS-1 when a low multiplicity of infection (less than 2) had been applied to them.
日本細菌学会の論文

Lactobacillus salivariusのテンペレートファージPLS-1による形質導入

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