乳酸菌に関する研究 : 腸内常在乳酸菌の混合培養

概要

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Each two species of the lactic acid bacteria of intestinal origin, Streptococcus faecalis, Lactobacillus acidophilus, and Lactobacillus bifidus, were mixed and cultured in vitro to pursue the occurrence of any interaction between the two.The growth rate of Sc. faecalis was not affected by L. acidophilus, but that of L. acidophilus was considerably repressed, although the same maximum bacterial number was reached eventually in the mixed culture as in the culture of L. acidophilus alone.The growth rate of L. acidophilus was not affected by L. bifidus, but that of L. bifidus was repressed by the same dose as, or a smaller dose of inoculum than that of L. acidophilus. Compatible growth occurred only when the doses of simultaneous inocuration of L. bifidus and L. acidophilus were at the rate of 104:103.When L. bifidus and Sc. faecalis were cultured together, growth of Sc. faecalis was generally rich, but that of L. bifidus was poor. Compatible growth was observed only when L. bifidus and Sc. faecalis were inoculated at the rate of 106:101, or when Sc. faecalis was inoculated about 15 hours after inoculation of L. bifidus. It was, however, worthy to note that a medium where Escherichia coli had been pre-cultured was found to allow a better growth of the two species tested than a original medium even if the two species were cultured in the same dose of inoculum and incubated for the same period.
日本細菌学会の論文