Pasteurella Multocidaの無細胞調製物によるタンパクへのアミノ酸のとりこみ-2-タンパクへのアミノ酸のとりこみ反応における終末産物の分析

概要

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C14-Labeled proteins obtained in the incorporation of C14-leucine into proteins by cell-free preparations from Pasteurella multocida, were analyzed and the following results were obtained.1) The radioactivity of C14-labeled proteins was not so greatly lowered by the treatment with alcali.2) More C14-leucine was released from C14-labeled proteins in acid hydrolysis of them with the increasing rate of hydrolysis.3) Many sorts of radioactive peptides were detected in the tryptic digest of C14-labeled proteins.4) The radioactivity of N-terminal or C-terminal amino acids of C14-labeled proteins was 0.5 or 30.5 per cent of the total, respectively.From these results it is concluded that the incorporation of amino acid into proteins by cell-free preparations from Pasteurella multocida was the incorporation into the internal linkage of peptide chains, namely due to the synthesis of peptides.
日本細菌学会の論文