土壌中の一桿菌が生産するぶどう球菌溶解性因子に関する研究
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The bacteriolytic agent for <I>Staphylococcus epidermidis</I> and some others was obtained from the culture filtrate of a newly isolated soil bacillus.The bacillus was similar to <I>Bacillus subtilis</I> morphologically.<BR>There have been many studies on bacteriostatic or bacteriolytic agent produced by a <I>B. subtilis</I> since subtilin. subtilin C, mycosutilin, subtenolin, subtilydin and endosubtilydin were reported by E. F. Jansen in 1944. The authors have also studied the properties of these organisms, the condition of lytic agent production and methods of purification. The results were as follows.<BR>1) The isolated organism, which was gram positive bacillus, grew well and formed a typical R. type colony on ordinary nutrient agar plate. The bacillus formed spore rapidly, fermented glucose, produced acid and gas and liquefied gelatin.<BR>2) Lytic agent affected <I>Staphylococcus, B. cereus, B. anthracis and B. subtilis</I>.<BR>3) The synthetic medium containing 1.0% glucose, 0.6% sodium-glutamate, 0.14% Na<SUB>2</SUB>HPO<SUB>4</SUB>, 0.1% KH<SUB>2</SUB>PO<SUB>4</SUB>, 0.2% NaCl, 0.01% MgSO<SUB>4</SUB>, adjusted to pH 6.0-7.2 was found to be the best for production of lytic agent. Incubation was usually continued for 6 days at 37°C.<BR>4) Physico-chemical properties of the lytic agent were relatively heat-stable, nondialysable through cellophane membrane and easily adsorbed on a strong acidic or basic ion exchanger column (IR 112, IR 120 or IR 400) but not adsorbed on active carbon. A purified fraction was obtained through Sephadex G-50 column.<BR>5) This agent acted on cell wall of several microorganisms at pH 5.0-6.0 and formed protoplast.
- 日本細菌学会の論文
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