コリスチンデスクによるコレラ菌とエルトール菌の鑑別

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Until recently, Greig test has been used as the most important method of differentiation between Vibrio cholerae and Vibrio eltor. In these several years, however, it has become difficult to depend only on this method for the differentiation of these two groups, since there has been found many strains of V. eltor which are negative in soluble hemolysine test. Therefore, several other methods have been reported by various workers for the rapid differentiation of both groups, one of these is the difference of sensitivity against polymyxin-B. It was found by the authors that the same difference of sensitivity is also observed with colistin, an antibiotic closely related to polymyxin-B, and we have devised a disk method using disk which contains a proper amount of colistin. This report is chiefly concerned with the experimental conditions of the test method.Results obtained are as follows:1) V. cholerae and V. eltor can be definitely differentiated by the sensitivity against colistin.2) Paper disks containing a proper amount os colistin can be used as the rapid and simple method for the differentiation of these two groups. V. cholerae forms a zone of inhibition of growth around the disk, whereas V. eltor forms no visible inhibitory zone.3) For the differentiation test, the disk containing 1, 000u of colistin methansulfonate at pH 6.0 is most excellent; the disk which contains 150u of colistin sulfate is also useful. The 50u disk of polymyxin-B is inferior to the above mentioned colistin disks for this purpose.4) Non-agglutinable vibrio showed the same response as V. eltor to colistin disk.
日本細菌学会の論文

著者

阿部久夫
横浜検疫所

コリスチンデスクによるコレラ菌とエルトール菌の鑑別

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