抗酸性菌による非特異的感染抵抗性に関する研究 : II. BCG菌体から抵抗性増強物質の分離について

概要

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Separation of active substance from BCG cells for heterologous immunity was tried.Free and bound-lipids were extracted by the method of Ledere and Asselineau. Thereafter, the defatted cells were treated first in the buffer of pH 4, then pH 7 and finally pH 9 for fractionation, followed by the treatment of Pronase-P. The enzyme treated bacterial residue was oxidized with NaIO4 and then reduced with NaBH4.The presence of heterologous immunogenicity in each fraction was tested, comparing with intact cells. Mice injected with Drakeol suspended fraction intraperitoneously were challenged with intravenous staphylococcal infection.Heterologous immunogenic activity was recognized in the residual fraction of NaIO4-NaBH4 treatment.The activity was also noted in phenol-extracted residue, urea extracted residue and cell wall. Through these finding, the heterologous immunogenic substance of BCG cells is different from the known lipopolysoccharide of Gram-negative bacteria or zymosan.
日本細菌学会の論文