鶏卵斜面培地培養BCGの室温保存に伴うrecoverbilityの推移

概要

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Four week old BCG culture on egg slant was preserved at room temperature and was examined on the recoverbility of BCG cells by checking their viability and stainability after various preservation periods. Estimation of the total and the assumably viable cell numbers per0.1ml was made by employing the authors' estimation formula and the stainability of the cells was examined by the malachitegreen-fuchsin staining.<BR>Results revealed that the decreasing trend of the recoverbility accompanied with the preservation period, i. e. the ratio between the actual viability and the estimated number of the recoverable cells, has shown lineality on the normal probability paper as the function of the square root of the preservation day. This straight line seems to be available for the estimation of the viability or the determination of the adequate concentration of the bacterial suspension to obtaincountable colonies from the egg slant cultures of BCG or tubercle bacilli preserved at room temperature for a rather long period of time.
日本細菌学会の論文