合成培地におけるVibrio El Torの溶血毒産生について

概要

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In the cultivation of El Tor vibrio Denken strain, in liquid media, the bacterial growth and hemolysin production are not parallal in various composition of culture media. In there sorts of synthetic media, Casamino acids medium, medium containing 18 amino acids, and medium containing 8 amino acids, 8 amino acids medium is best in the hemolysin production per unit amount of cells. To investigate the mechanism of this phenomenon, the Fe content of these media was measured according to the O-phenanthroline method; but, the difference of Fe content in these three media was not so definite as to explain the reason of above mentioned phenomenon. Then, after removing the total Fe in the three sortes of media, FeSO4·7H2O was added in various amount to these media, and relation between the bacterial growth and hemolysin production was investigated, but the effect of Fe on the hemolysin production was not clear; the addition of FeSO4·7H2O in 50 γ/ml to 8 amino acids medium seemed somewhat helpful for the hemolysin production.
日本細菌学会の論文