Establishment of a human gingival carcinoma cell line growing in a serum-free medium and studies on its application. 1. Establishment of human gingival carcinoma cell line (Ca 9-22 SF) growing in serum-free medium.:1. Establishment of human gingival carci

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概要

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• It is conceived that serum-free culture is very useful in investigating the mechanism of cell differentiation and growth, or in studying the substance which affects them. It was attempted to culture the gingival carcinoma cell (Ca 9-22) in a serum-free condition to investigate the substance which affected the growth and differention of the oral cancer. Ca 9-22 SF was established as a result, which was grown in long-term culture in the serum-free defined medium. Subculture of Ca 9-22 SF was usually performed once every two weeks using trypsin and soybean trypsin inhibitor. Ca 9-22 SF grew 2 days after inoculation and the doubling time at exponential growth phase was estimated to be about 18 hours which was the same as Ca 9-22, but the achieved final cell concentration was lower than that of Ca 9-22. In the chromosomal analysis, the patterns of chromosomal distribution of both cell lines were similar and both modes were 59. The marker chromosome of Ca 9-22 SF was undetectable. Ca 9-22 SF was transplantable to nude mice but the carcinogenetic efficiency in mice (2/6) was lower than that of Ca 9-22 (5/6). The formed nodule was histologically determined as squamous cell carcinoma but was well differentiated in contrast to that of Ca 9-22 which was moderately differentiated. Ca 9-22 SF is considered to maintain the character of the oral cancer cell and is able to grow in the defined medium free of serum, hormones or growth factors, so that it is expected to be used in studying the effects of various substances on the growth and the differentiation of the oral cancer.

• 社団法人 日本口腔外科学会の論文

著者

• 力丸 浩一

  東京医科歯科大学歯学部第2口腔外科学教室

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