グリオキサラ-ゼ1活性に及ぼすフラボノイドの影響

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The influence of 19 flavonoids (ten flavonols, two flavanones, one flavone and six isoflavones) was investigated on glyoxalase I [S-lactoylglutathione methylglyoxal-lyase (isomerizing) EC 4. 4. 1. 5] activity from yeast and bovine liver. The enzyme activity was estimated from increments of absorbance at 240 nm in produced S-lactoylglutathione. Quercetin, fisetin, gossypitrin and my-ricitrin showed strong inhibition at 0.1mM. Inhibitory activities of isoflavones were weak to slight. In general, inhibitions to yeast enzyme were somewhat greater than to bovine liver en-zyme. The concentration of quercetin and fisetin which produced 50% inhibition (I50) to yeast enzyme was 30 and 15 μM, respectively; I50 of quercetin to bovine liver enzyme was 36 μM. The mode of inhibition of yeast enzyme by quercetin was competitive with a Ki value of 22μM. The inhibition of yeast enzyme by either quercetin or morin was not reversed by the presence of excess albumin from egg white or bovine serum.