下痢性および麻痺性ホタテガイ貝毒の微生物的定量

概要

論文の詳細を見る
Two bacterial strains the growth of which was inhibited by extracts of the hepatopancreas of scallops, Patinopecten yessoensis, were isolated from soil and identified as being Bacillus spp. The growth of both strains was inhibited by the addition of okadaic acid or saxitoxin to the medium. The growth of Bacillus OH-D decreased to 30% with 10 μg of okadaic acid per 10ml of broth, and was inhibited completely with 25 μg of this acid per 10ml of the broth. There were clear growth-inhibition zones around cylinders containing saxitoxin on a carpet of Bacillus OH-P with the width of the zone proportional to the concentration of saxitoxin. Crude and partially purified fractions of hepatopancreas containing diarrhetic or paralytic shellfish toxins inhibited the growth of strains OH-D or OH-P, respectively. Free fatty acids in the hepatopancreas did not interfere with the inhibition of growth by these toxins. Our findings suggested microbial titration could be used to assay shellfish toxins instead of assays of lethality in mice.
社団法人日本農芸化学会の論文