茶葉のリボヌクレア-ゼ(RNase2)による2′-ヌクレオチドの生成について

概要

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A characteristic ribonuclease (RNase 2) was partially purified from the extracts of tea leaves by means of column chromatography on DEAE-cellulose and Sephadex G-100. By the action of this enzyme, yeast RNA gave three kinds of 2'-mononucleotides (2'-AMP, 2'-GMP and 2'-UMP) together with oligonucleotides, and accumulation of intermediate 2', 3'-cyclic nucleotides was not detected in the hydrolysate. However, it was found that four kinds of 2', 3'-cyclic nucleotides were very rapidly hydrolyzed to the 2'-nucleotides and cleavage rate of the phosphodiester linkage of 2', 3'-cyclic AMP was 250 times grater than that of adenylyl-(3'→5')-adenosine. From the properties of the RNase 2, it is considered that 2'-nucleotides in black tea are produced by the action of this enzyme.
社団法人日本農芸化学会の論文