Asporgillusによるトリプトファンからカテコールの生成

概要

論文の詳細を見る
In previous paper, isolation and identification of catechol from shoyu was reported. In this paper, the formation of catechol was studied. The results were as follows. 1) When Aspergillus soyae (KS) was cultured for 7 days at 30°C on Czapek solution containing 0.1% tryptophan, 2, 3-dihydroxybenzoic acid and catechol were formed from tryptophan. 2) 2, 3-Dihydroxybenzoic acid was isolated from Czapek solution containing 0.1% tryptophan cultured by Aspergillus soyae (KS). 3) Catechol was isolated from Czapek solution containing 0.1% 2, 3-dihydroxybenzoic acid cultured by Aspergillus soyae (KS). 4) Productivity of 2, 3-dihydroxybenzoic acid and catechol from tryptophan was found in strains of Aspergillus. 5) By resting cell culture of Aspergillus soyae (KS) in 1/15M phosphate buffer (pH 5.5), 2, 3-dihydroxybenzoic acid and catechol were formed from tryptophan, kynurenine, and anthranilic acid but not formed from kynurenic acid, 3-hydroxyanthranilic acid and o-aminophenol. 6) From these results, we recognized the pathway for the formation of catechol in shoyu via kynurenine, anthranilic acid and 2, 3-dihydroxybenzoic acid from tryptophan in Aspergillus.