発芽植物の物質代謝 : (第4報)クエン酸生成反応

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Previously we obtained some evidences that the enzymatic synthesis of citric acid of plant tissues was dependent on CoA as it was confirmed in animal or microbial tisssues. Additional results are given in the present paper indicating that the citrate synthesis in mung bean or soy bean from pyruvate and oxalacetate should proceed through reaction (I) and (III). The reaction sequence was verified as follows: (I) Addition of catalytic amounts of CoA was effective in the synthesis of citrate from py-ruvate and oxalacetate by twice-washed particulate enzyme (presumably mitochondria) of mung bean or soy bean seedlings as shown in Table 1. (II) The plant enzyme was devoid of acetate activating activity even in the preesnce of CoA, but in combination with E. coli preparations, it actively catalyzed the citrate synthesis from acetate, oxalacetate, CoA, and ATP (reaction IIb and III). The results can be taken as an indirect evidence for the presence in the plant preparations of “condensing enzyme” which catalyzes the synthesis of citrate from acetyl-CoA and oxalacetate. (Table 2).
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