PepsinのPeriodateによる酸化
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概要
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In this experiment pepsin was oxidized by natrium metaperiodate under the various experimental conditions, and the following results were obtained through the investigating the process of oxidation and the changes of enzymatic activity and of ultraviolet absorption. 1) The enzymatic activity decreases as the reaction of oxidation advances. 2) About 30 moles of periodate react with 1 mole of pepsin. 3) In parallel with the decreasing of enzymatic activity during oxidation, extinction cofficient at 277mμ lowers. This lowering of the optical density is chiefly caused by decomposition, and it is concluded that tryptophan also seems to play an important rAle, to peptic activity.
- 社団法人 日本農芸化学会の論文