酸化α-Amylaseに関する免疫学的研究
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概要
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A certain amount of amylase is contained usually in serum and urine as normal constituent, and in a disease, e.g., acute pancreatic necrosis, it increases enormously. But there is no evidence to indicate the fact that any anti-enzyme against amylase is formed. As amylase by the body is excreted into blood from glandular cells of pancreas by some mechanism, it is physiologically a matter of course that it should not form antibody in the body. Amylase differs in its origin. Therefore, it should be assumed, that just as, for instance, anti-sera against foreign enzymes are produced, so antibody against amylase of foreign origin is also produced in the animal body. Up to this time, there have been many reports about anti-amylase, but because the samples used are impure, it is doubtful whether antibodies against enzyme or those against contaminated impurity are produced. By the use of crystalline α-amylase originated from bacteria and of crystalline oxi.-α-amy lase prepared by periodate and still retaining a part of activity, we have tried the experiment to clarify the question whether or not the active surface as enzyme and the determinant group combining with anti-sera are one and the same. The results. are summarized in tables. They lead to the conclusion that antibody against bacterial α-amylase is never produced in the rabbits body. But unexpectedly antibody aginst oxi. -α-amylase is produced and this anti-sera shows precipitation with not only oxi. -α-amylase, but also a-amylase. When the ratio of antibody to antigen is low, the precipitated complex has a little of enzymatic activities, but in the case of high ratio, it doesnt show any action. From these facts, it is assumed that anti-sera, formed by sensitization with oxi.-α-amylase, combines with the active surfaces of α-amylase and oxi.-α-amylase, to show precipitation.
- 社団法人 日本農芸化学会の論文